Imagine this: you’ve spent months nurturing a batch of premium cannabis clones, hand-selected from a trusted source. They’re vigorous, vibrant, and primed for a bumper harvest. But when flowering kicks in, disaster strikes: the plants stall out, the buds turn into small, sad, airy little balls, and your yields crash by up to 50%. Welcome to the world of Hop Latent Viroid (HpLVd), the invisible destroyer that has become the cannabis grower’s worst nightmare. Often dubbed the “COVID of cannabis”, HpLVd doesn’t just hit hard; it spreads silently through your clones, underscoring why plant health is non-negotiable for any grower.
In this guide, we’ll demystify plant viruses (and viroids like HpLVd) in plain English—think science served with a touch of grow-room wisdom. We’ll trace the stealthy history of HpLVd, highlight its red flags, and arm you with battle-tested strategies to prevent and fight it. Whether you’re a seasoned grower or just dipping your toes into cloning, this is your roadmap to resilient, virus-free crops. Let’s dive in.

Hop Latent Viroid – HpLVd
Plant viruses 101: Nature’s saboteurs
Before we zoom in on HpLVd, let’s pull back and look at the bigger picture: plant viruses and viroids. These tiny lurkers are the ultimate uninvited guests in your garden, but understanding them is your first line of defence.
Cannabis viruses
At their core, plant viruses are fragments of genetic code—either RNA or DNA—wrapped in a protein coat (or not, in the case of viroids). They’re not alive the way bacteria are; they’re more like rogue software that needs a host computer (your plant’s cells) to run. Once inside, they hijack the cell’s machinery: the plant’s own ribosomes and enzymes are pressed into service to crank out viral copies instead of essential plant proteins. This replication frenzy drains resources, disrupts metabolism, and triggers weird growth patterns.
Think of it like this: a virus sneaks into your plant’s “factory” (the cell nucleus or cytoplasm) through a back door—a pruning wound, an insect bite, or even contaminated tools. It tricks the factory workers (enzymes) into building a viral assembly line, flooding the system until cells burst or start sending distress signals to their neighbours. The result? A cascade of chaos.
Viroids, the category HpLVd belongs to, are even more stripped-down villains: naked loops of RNA with no protein coat, only 250–400 nucleotides long. They’re the minimalist hackers—small enough to slip past defences and replicate using the plant’s nuclear machinery via a “rolling-circle” method, spitting out error-prone copies that evolve on the fly.
Transmission is their superpower. Unlike animal viruses that travel by air or touch, plant viruses rely on vectors:
- Mechanical spread: sap from infected plants on your scissors or hands gets transferred to healthy ones during cloning or trimming.
- Insect messengers: aphids, whiteflies, or leafhoppers sip virus-laden sap and deposit it elsewhere.
- Vegetative betrayal: grafts, cuttings, or clones carry the load directly from the mother plant to the babies.
- Seed surprises: rare, but some viruses travel in pollen or embryos.
Symptoms vary by virus and host, but often include:
- Mosaic patterns: mottled, yellow-green leaves that look like a bad paint job.
- Stunting: dwarfed growth, as if the plant hit a permanent growth plateau.
- Necrosis: dead spots or wilting, the plant’s SOS flare.
- Yield impacts: fewer flowers, smaller fruits, or watered-down potency—devastating for cannabis.
The catch? Many infections lurk asymptomatically, only showing up under stress like heat, drought, or flowering. For growers, that means vigilance: healthy plants fight back better, so balanced nutrition, optimal light, and low-stress environments are your baseline armour. Now, let’s meet the viroid that’s driving the cannabis world crazy.
HpLVd: From hop fields to cannabis chaos—a brief history
Hop Latent Viroid earned its name from humble beginnings in hop yards. Discovered in 1987 in Spain, it first appeared as an innocuous RNA oddity in Humulus lupulus (hop, the cannabis plant’s botanical cousin in the Cannabaceae family). By 1988, surveys in Germany revealed it was infecting 90–100% of European hop cultivars—yet hops largely shrugged it off with mild symptoms, like a modest drop in cone yields (8–37%) or bitter acids (15–50%). Brewers noticed subtler beer flavours from altered terpenes, but there was no industry-wide panic.
Fast-forward to the cannabis boom. HpLVd jumped species around 2017, probably via shared propagation tools or infected germplasm in U.S. facilities. The first rumblings showed up on online forums in 2014, with growers complaining about the “dudding disease”—stunted, brittle plants producing airy buds. By 2019, high-throughput sequencing nailed it: HpLVd was the culprit in California, where a survey by Dark Heart Nursery estimated 90% of operations were contaminated. It then tore through North America, Canada, and beyond, with infection rates averaging 30% across the industry and economic hits nearing $4 billion annually in lost yields and potency.
Why cannabis? Unlike resilient hops, weed is a softer target. HpLVd’s 256-nucleotide circular RNA thrives in cannabis cells, replicating in the nucleolus and clogging up metabolite production. It hits glandular trichomes especially hard, slashing THC by 50–70%, terpenes by up to 40%, and overall vigour. Two variants (Can1 and Can2) have adapted, with mutations like U225A boosting infectivity. It’s pleiotropic—symptoms range from none to full-blown nightmare—making it a shapeshifter in your grow.
Bottom line, HpLVd’s “latent” label is a lie in cannabis: it hides in veg, then explodes in flower, turning premium clones into liabilities.
Spotting the cannabis virus: HpLVd symptoms in your grow

Electron microscope image of cannabis trichomes. The trichomes on the left are stunted and smaller due to infection with Hop Latent Viroid. Right: healthy trichomes. Image source: Simon Fraser University, Canada
Early detection is your crop’s guardian angel, but HpLVd plays hide-and-seek like a pro. In clones from infected mothers, it often lies dormant until week 4+ of flower, when stress unmasks it. Here’s what to watch for, especially if you run a clone-heavy setup:
- Stunting and structural changes: shorter internodes, more horizontal sprawl than vertical reach, and overall dwarfing—as if the plant were stuck in permanent juvenile mode.
- Brittle stems and leaves: they snap like dry twigs; foliage yellows (chlorosis) or curls unevenly.
- Dudding disaster: the hallmark—buds stay small, loose, and sparse. Trichomes ripen early (amber too soon), resin production tanks, and aromas fade.
- Potency crash: lab tests show cannabinoid drops (THC down 50%+), terpene loss (myrcene oddly elevated, β-caryophyllene down 13–29%), and weaker flavour.
Not all cultivars respond the same way; some coast with few symptoms while others fail en masse. Co-infections (e.g., with other viroids) amplify the damage. Pro tip: scout weekly under magnification—uneven trichome distribution is a screaming red flag. When in doubt, test: RT-PCR on leaf samples from both old and new growth is the gold standard, catching around 30% of silent carriers.
Transmission traps: Why clones are HpLVd’s highway
HpLVd doesn’t fly or float; it’s a full-contact sport. As a viroid, it needs direct sap-to-sap transfer—perfect for clone-heavy operations.
- Clone-borne contagion: the big one. Infected mother plants pass it 100% to cuttings. One sketchy clone in your tray? Boom—your whole batch is toast.
- Tool terrorism: pruners, scalpels, or gloves smeared with sap spread it like wildfire. Recirculating hydro systems or shared reservoirs amplify the problem.
- Human highways: workers touching multiple plants without washing? Instant vector.
- Rare routes: pollen/seed transmission is negligible; no known insect vectors.
In cannabis, where 70%+ of crops start from clones, this transmission chain explains the explosion. A single imported cutting can doom an entire facility. Lesson: plant health starts upstream—vet your sources ruthlessly.
How to prevent it: Protect your clones from day one
Good news: HpLVd is beatable with prevention. Focus on clean inputs and rock-solid hygiene—your clones will thank you.
- Source smart: ditch untested clones; opt for certified clean stock or start from seed (much lower risk). Quarantine new arrivals for 30 days, testing in week 3 via lab RT-PCR or dot-blot.
- Sanitise like a surgeon: bleach (5–10% sodium hypochlorite) or Virkon S (2%) on tools—alcohol doesn’t cut it, since it precipitates RNA. Heat-treat blades at 160°C for 10 minutes. Swap PPE between plants; wash hands obsessively.
- Segment your grow: keep veg and flower separated; use dedicated cloners per batch. Filter water and avoid runoff mixing.
- Boost resilience: healthy plants fight back via RNA silencing. Dial in balanced nutrients at the right pH, stable temps, and low stress—strong clones slow viral buildup.
- Test religiously: sample 10–20% of your stock every quarter. Early wins save whole harvests.
These steps cut risk by about 90%—proven in hop yards and cannabis labs alike.
Fighting an outbreak: Damage control when HpLVd hits
Found an infection? Don’t panic—move fast. There’s no silver-bullet antiviral, but here’s your playbook:
- Cull ruthlessly: chop symptomatic plants immediately; burn or bleach waste to kill persistent RNA.
- Rescue team: for salvageable mothers, try meristem-tip culture (micro-propagating tiny <0.5 mm shoot tips) paired with cold (2–4°C for months) or heat therapy (36°C for 2 weeks). This can knock down viral load via mutations but it’s not foolproof—re-infection is a constant threat.
- Facility wash-down: deep-clean everything; use urea or chloropicrin for soil. Restart with verified clean material.
- Long-term R&D: breed resistant cultivars or deploy RNA interference—emerging tools, but not quite ready for your grow room yet.
Recovery hurts yields in the short term, but rebuilds trust in your system. Remember: one clean cycle resets the clock.
Final harvest: Put plant health first for thriving grows

Comparison between a healthy plant (right) and a plant affected by HLVd (left), where the viroid’s influence on trichome production is clearly visible. Source: Dark Heart Nursery
HpLVd isn’t just a viroid—it’s a wake-up call. In an industry hooked on clones for speed and uniformity, its spread drives home why plant health trumps everything. From virus basics to outbreak ops, once you’re armed with the right knowledge, you can grow with confidence, dodge duds, and deliver your best.
At Alchimiaweb, we aim to empower growers by giving them the tools to succeed. Stock up on sterile gear, test kits, or clean genetics today—your next round of clones is waiting. Got HpLVd stories or tips to share? Drop them in the comments. Grow Happiness!
Bibliography
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- Adkar-Purushothama, C. R., Sano, T., & Perreault, J. P. (2023). Hop latent viroid: A hidden threat to the cannabis industry. Viruses, 15(3), 681. https://doi.org/10.3390/v15030681
- Punja, Z. K., Collyer, D., Scott, C., Holmes, J., Zhao, Y. Y., Hinz, F., … & Reed, S. (2023). Symptomology, prevalence, and impact of hop latent viroid on greenhouse-grown cannabis (Cannabis sativa L.) plants in Canada. Canadian Journal of Plant Pathology, 46(2), 174–197. https://doi.org/10.1080/07060661.2023.2279184
- Punja, Z. K., Scott, C., Tso, H. H., Munz, J., & Buirs, L. (2025). Transmission, spread, longevity and management of hop latent viroid, a widespread and destructive pathogen affecting cannabis (Cannabis sativa L.) plants in North America. Plants, 14(5), 830. https://doi.org/10.3390/plants14050830
- Puchta, H., Ramm, K., & Sänger, H. L. (1988). The molecular structure of 26 S rRNA from Humulus lupulus L. (hops) and the sequence of a viroid-like RNA associated with hop stunt disease. Nucleic Acids Research, 16(9), 4197–4216. https://doi.org/10.1093/nar/16.9.4197
- Warren, J. G., Mercado, J., & Grace, D. (2019). Occurrence of hop latent viroid causing disease in Cannabis sativa in California. Plant Disease, 103(10), 2699. https://doi.org/10.1094/PDIS-03-19-0530-PDN
- Viruses and Viroids – an overview. (n.d.). In ScienceDirect Topics. Elsevier. Retrieved November 24, 2025, from https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/viruses-and-viroids
- Hop latent viroid in hemp. (n.d.). OSU Extension Service. Oregon State University. Retrieved November 24, 2025, from https://extension.oregonstate.edu/catalog/em-9570-hop-latent-viroid-hemp
- Kovalchuk, I., Pellino, M., Rigault, P., van Velzen, R., Bhalerao, R., Clark, J., … & Kovalchuk, A. (2020). The Genomics of Cannabis and Its Conservation. Genome Biology and Evolution, 12(3), 292–312. (For general viroid context; referenced in broader reviews)
- Hop Latent Viroid: A Guide to Sampling, Testing and Lab Selection. (2024, July 9). Cannabis Business Times. Retrieved November 24, 2025, from https://www.cannabisbusinesstimes.com/disease/cannabis-plant-disease/news/15686586/hop-latent-viroid-a-guide-to-sampling-testing-and-lab-selection
- Bektaş, M., Sõmera, M., Faggioli, F., & Pallas, V. (2019). First report of hop latent viroid on marijuana (Cannabis sativa) in California. Plant Disease, 103(10), 2699. https://doi.org/10.1094/PDIS-03-19-0530-PDN
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